Artificial M a I N T E N a N C E Media for Cell and Organ Cultivation I. the Cultivation of Fibroblasts in Artificial and Serumless Media* by Lillian

The importance of developing artificial media that can be used in the place of serum for maintaining the life of tissues and organs outside the body hardly needs to be emphasized. Many of the studies for which the organ culture technique 1 was developed, as well as others that can be carried on by the simpler methods of tissue culture, depend for their success on the creation of suitable artificial media. These media are needed to reduce the cost of experimentation, to make possible extensive cultivation of human organs and those of small animals from which serum in sufficient quantity cannot be obtained, and for all studies in which the production of serum and other protein substances is to be investigated. For the latter purpose, media that are serumless and free from protein will be required. But for other work media that contain serum as one constituent may be used. Several artificial media designed to promote rapid growth of cells in tissue culture have already been described3 ,3 But these are not